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Gel Pro 6.0	Array Pro	Compare Software

	Ge Pro 3.1	Gel Pro 4.5	Gel Pro 6.0
General Image Processing & Analysis Tools
Image/experiment database	∨	∨	∨
Extensive image processing and handling (based on Image-Pro Plus)	∨	∨	∨
New spatial calibration techniques			∨
Distance measurement tools			∨
Multiple color channel format handling			∨
Create color composite images			∨
Sharpen, emboss and starfield subtraction tools			∨
Local zoom tool provides a magnified area of your image in a second window			∨
Synchronized pan and zoom tool lets you simultaneously explore multiple images			∨
Rotation of image for best lane/band tracking accuracy	∨	∨	∨
Macro recording and programming (basic-like)	∨	∨	∨
Macro player to view Gel-Pro macros			∨
Intensity and spatial calibration	∨	∨	∨
Non-destructive annotation overlay		∨	∨
3D surface plot to aid data interpretation		∨	∨
Full support for acquisition of sequences and extraction of frames for analysis		∨	∨
Undo buffer		∨	∨
Recovery of analysis progress from inadvertent computer power loss		∨	∨
Support for the GLP/CRF 21 Part 11 Compliance using audit trail and image/file signature		∨	∨
Operator identification management based on NT log-in			∨
As part of the annotation toolbar, fields appear for date, time, and filename		∨	∨
Tooltips are enabled to give information known about lanes, bands, dot/blot spots or area density spots		∨	∨
Lanes
Automatic lane recognition	∨	∨	∨
Recognizes and tracks curved lanes	∨	∨	∨
Editable lane definition	∨	∨	∨
Lane annotation and ID	∨	∨	∨
Option for non-uniform lane width; individual lanes may be different widths		∨	∨
Active lane in profile is outlined boldly both on the image and the profile which aids the user when many lanes are displayed		∨	∨
Ability to define variable well origin for 1D analysis		∨	∨
Bands			∨
Automatic band recognition	∨	∨	∨
Add/delete bands on image or profile	∨	∨	∨
Display bands by extent or crosshair indication	∨	∨	∨
Band annotation	∨	∨	∨
Interactive band height sensitivity to aid in proper automatic band finding		∨	∨
Define band pattern (grimace) to achieve better band resolution for closely-spaced, distorted bands		∨	∨
ID by band position within lane or by rows of equal Rf or MW		∨	∨
Band extents may be edited via handles on the profile	∨	∨	∨
Band extents may be edited via handles on the image overlay		∨	∨
User may select position for band labels for optimum clarity		∨	∨
Ability to perform a fourth order polynomial best fit warping for user selected bands that are interpolated between defined bands) through the rest of the lane		∨	∨
Lane Profile
Show single or multiple profiles	∨	∨	∨
Bands may be indicated by peak position or center of mass	∨	∨	∨
Lane bitmap may be displayed under the profile to aid interpretation		∨	∨
Bands may be indicated by brackets, dotted lines or cross-hatching		∨	∨
Band indicators are arrows to pinpoint band position		∨	∨
X and Z scale zooming for close examination		∨	∨
Easy cycling and selection of the lanes via the profile dialog display		∨	∨
Background			∨
Flat offset	∨	∨	∨
Extract from background	∨	∨	∨
Valley-to-valley	∨	∨	∨
Filtered profile	∨	∨	∨
Rolling disk		∨	∨
Option to subtract background for profile display	∨	∨	∨
Option to display image with background subtracted to let the user visualize whether a correct background method has been applied		∨	∨
Interactive feedback of results as the methods and parameters are changed to optimize results		∨	∨
Quantitation
Amount may be determined by the area in the profile under the entire profile or only the area within the bands		∨	∨
Assign quantitation relative to amount loaded on the lane	∨	∨	∨
Ratio to single band, band row or lane	∨	∨	∨
Build calibration curve fit: first - third order polynomial and Lagrange	∨	∨	∨
Multiple amount calibration curves can be defined and used on a single image		∨	∨
Results Table
Show MW, amounts or both		∨	∨
Show amounts, relative amounts, %, IOD or Max OD		∨	∨
When bands, lanes or rows are selected, the corresponding elements on the image are outlined boldly to aid user interpretation		∨	∨
The row band graph shows a bar chart of the amounts within a row across each lane		∨	∨
Presence/absence may be indicated matched to lane 1 via 0, 1, -1 assignment for easy typing		∨	∨
Molecular Weight
Slant correction to correct gel warping and uneven band migration	∨	∨	∨
Multiple MW standard lanes with auto-slant correction	∨	∨	∨
Hyperbolic cosecant curve fitting produces more accurate MW calculation for small DNA fragments (under 1,000 base pairs) run in polyacrylamide		∨	∨
Polynomial slant fit lines may be specified to follow uneven migration more closely and give more accurate results		∨	∨
Dot/Blot
Identify regular or irregular layouts of regular spots	∨	∨	∨
Quantitate by ratio methods	∨	∨	∨
Quantitate by calibration curve methods	∨	∨	∨
Three background determination options	∨	∨	∨
Auto or manual thresholding	∨	∨	∨
Choose elliptical or rectangular spot designation	∨	∨	∨
Replicate sampling data analysis	∨	∨	∨
User may ask to have missing spots filled in automatically for a regular array when all dots are not found		∨	∨
An area filter tolerance may be specified to sort out actual spots from background contamination		∨	∨
Area Density
Free form or auto-trace definition of spots of interest		∨	∨
Extensive table of measurements and measurement statistics		∨	∨
Colony Counting
Full complement of automatic and manual spot finding		∨	∨
User may edit, add, split, merge spots		∨	∨
Numerous filtering and measurement options		∨	∨
Extensive table of measurements and measurement statistics		∨	∨
Automatic classification by user criteria		∨	∨
Collaborate & Share
Output graphic windows to printer, clipboard, or underlying data	∨	∨	∨
Output data windows to printers, text, DDE to Excel, copy to clipboard, append or to data file	∨	∨	∨
Internet remote file transfer	∨	∨	∨
Extensive print layout and control, including poster print	∨	∨	∨
Report generator	∨	∨	∨
File & System Support
Updated driver support			∨
Dye and lens file support			∨
Extensive file format input/output, including: TIFF, HPP, BMP, CUT, EPS, JPG, Photo CD, PCT, PCX, TGA, and FLAT (Binary)	∨	∨	∨
JPEG, LZW, RLE compression supported	∨	∨	∨
JPEG 2000 read and write file support			∨
Extensive library of camera/scanner acquisition (based on Image-Pro Plus library)	∨	∨	∨
Supports 8, 12, 16, 24, and 32 bit RGB images; 8, 12, 16 bit grayscale images	∨	∨	∨
Supports 48 bit RGB point images		∨	∨
Operating Systems
Win 95	∨		∨
Win 98 SE	∨	∨	∨
Win 98 ME	∨	∨	∨
Win NT 4 SP 6a	∨		∨
Win 2000	∨	∨	∨
Win XP Professional	∨	∨	∨